Part:BBa_K4796003:Design

Strong 5'UTR for Vibrio natriegens (BBa_K3187014 derivative)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part was designed to combine known sequence features that enhanced transcription from T7 RNA polymerase and translation from ribosomes in Vibrio natriegens. Design started with BBa_K3187014 which had previously been designed for optimal expression in E. coli, and sequence features improving transcription/translation by T7 RNAP in Vibrio natriegens were added.

Source

Modified from BBa_K3187014 with artificial sequences and a 9 bp linker from Bacillus methanolicus.